I got the topic of my final thesis this morning.
Antimicrobial peptides: its gene transduction and expression in prokaryotic and eukaryotic host system.
“You can try the prokaryotic host–vector system at first, for the eukaryotic part is more difficult.”
My teacher said to me.
Well, so I will try my best.
Although I know something about antimicrobial peptide, to get an accurate definition I browsed WIKI.
Antimicrobial peptides: its gene transduction and expression in prokaryotic and eukaryotic host system.
“You can try the prokaryotic host–vector system at first, for the eukaryotic part is more difficult.”
My teacher said to me.
Well, so I will try my best.
Although I know something about antimicrobial peptide, to get an accurate definition I browsed WIKI.
Antimicrobial peptides (also called host defence peptides) are an evolutionarily conserved component of the innate immune response and are found among all classes of life.
These peptides are potent, broad spectrum antibiotics which demonstrate potential as novel therapeutic agents. Antimicrobial peptides have been demonstrated to kill Gram negative and Gram positive bacteria (including strains that are resistant to conventional antibiotics), mycobacteria (including Mycobacterium tuberculosis), enveloped viruses, fungi and even transformed or cancerous cells. Unlike the majority of conventional antibiotics it appears as though antimicrobial peptides may also have the ability to enhance immunity by functioning as immunomodulators.
So that this topic seemed very interesting, for it would be the first time I touch a whole process about molecular biological experiment, I felt little nervous. Refer to molecular biological experiment; I have to think it is a time-consuming and sorta uncertain work, is not it? In all the steps, you can not see any thing which you want to get: DNAs, RNAs, some dissoluble proteins, or something else. So you do not know whether you get them definitely.
In my experiment, I think the most difficult part should be “primer design” and “vector build.” As soon as my design was correct and I build good vectors, there were not so hard of its transduction and expression. Another, during my experiment, I will try some different DNA donors – a contrast test? Yes, maybe it is!
Failed and try again, time by time. The research road is not plain. Nevertheless, I will make out it, and my final thesis experiment is my first signpost.
In my experiment, I think the most difficult part should be “primer design” and “vector build.” As soon as my design was correct and I build good vectors, there were not so hard of its transduction and expression. Another, during my experiment, I will try some different DNA donors – a contrast test? Yes, maybe it is!
Failed and try again, time by time. The research road is not plain. Nevertheless, I will make out it, and my final thesis experiment is my first signpost.